Draize Eye Irritancy Test Rabbits
ثبت نشده
چکیده
Household products, shampoos, pesticides, weed killers, even riot control gases, are just some of the products tested for irritancy in the eyes of conscious rabbits. The Draize test, introduced in 1944, is cruelly simple. The substance under test is sprayed or instilled into one eye of an albino rabbit. Generally no pain relief is given and the test often proceeds for 7 days during which the cornea, iris and conjunctivae are examined for signs of opacity, ulceration, haemorrhage, redness, swelling and discharge.
منابع مشابه
An in Vitro Evaluation Method to Test Ocular Irritation using a Human Corneal Epithelium Model
Recently, safety evaluation tests that do not involve animal experiments have been prosperously developing. However, the optimal evaluation materials and methods for assessing ocular irritancy have not been well investigated. In this study, we determined the optimal evaluation method for testing ocular irritation using a human cultured corneal epithelium model (corneal model). In order to asses...
متن کاملColworth, United Kingdom as an in vitro alternative to the in vivo Draize rabbit eye test method for the assessment of eye irritation
The IRE test was developed by Burton and his colleagues at Unilever Research Laboratory, Colworth, United Kingdom as an in vitro alternative to the in vivo Draize rabbit eye test method for the assessment of eye irritation (Burton et al. 1981). In the IRE test method, liquid test substances are spread using a syringe and solids are pulverized and applied as a powder over the corneas of enucleat...
متن کاملThe Isolated Chicken Eye test to replace the Draize test in rabbits.
In 1944, Draize et al., published a paper entitled "Methods for the study of irritation and toxicity of substances applied topically to the skin and mucous membranes". The Organization for Economic Co-operation and Development published their first guideline on eye irritation in 1981, using rabbits. In the early eighties the development of alternative non-animal tests to replace the Draize eye ...
متن کاملAlternatives to In Vivo Draize Rabbit Eye and Skin Irritation Tests with a Focus on 3D Reconstructed Human Cornea-Like Epithelium and Epidermis Models
Human eyes and skin are frequently exposed to chemicals accidentally or on purpose due to their external location. Therefore, chemicals are required to undergo the evaluation of the ocular and dermal irritancy for their safe handling and use before release into the market. Draize rabbit eye and skin irritation test developed in 1944, has been a gold standard test which was enlisted as OECD TG 4...
متن کاملResazurin metabolism assay is a new sensitive alternative test in isolated pig cornea.
The main object of our study was to investigate whether the resazurin metabolism assay is a sensitive surfactant and alcohol toxicity test in isolated pig cornea and to compare this recently developed fluorometric assay with the data collected in the eye irritation reference chemical data bank. Resazurin is a substrate that changes color in response to metabolic activity. Isolated pig corneas w...
متن کامل